HDGF2 (Cryptic Specific) Antibody #95895
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 135 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
HDGF2 (Cryptic Specific) Antibody recognizes endogenous levels of total HDGF2 cryptic protein. This antibody does not detect wild-type HDGF2 protein.
Species Reactivity:
Source / Purification
Background
Exon splicing of HDGF2 mRNA is regulated by TAR DNA-binding protein 43 (TDP43), a ubiquitously expressed DNA/RNA-binding protein belonging to the heterogeneous nuclear ribonucleoprotein (hnRNP) family. Dysfunctional TDP43, driven by genetic mutations, hyperphosphorylation, ubiquitination, and cleavage forms insoluble cytoplasmic aggregates. These aggregates are pathological hallmarks of patients with amyotrophic lateral sclerosis (ALS), frontotemporal lobar degeneration (FTLD), and limbic predominant age-related TDP-43 encephalopathy (LATE). These inclusions are also present in some patients with other neurodegenerative diseases (NDDs), including Alzheimer’s disease (AD) and Chronic traumatic encephalopathy (CTE) (4,5). TDP43 cytoplasmic mislocalization and aggregation lead to altered HDGF2 mRNA splicing and the inclusion of cryptic exons. This results in an alternative translated HDGF2 protein that includes cryptic exon-encoded neo epitopes. Neo epitope-containing HDGF2 is detected in CSF and plasma, suggesting it may be a biomarker for neurodegenerative diseases with TDP43 pathology (6,7).
- Baude, A. et al. (2016) Nucleic Acids Res 44, 2214-26.
- LeRoy, G. et al. (2019) Sci Adv 5, eaay3068.
- Albagli, E.A. et al. (2024) Mol Neurodegener 19, 79.
- Jo, M. et al. (2020) Exp Mol Med 52, 1652-1662.
- de Boer, E.M.J. et al. (2020) J Neurol Neurosurg Psychiatry 92, 86-95.
- Agra Almeida Quadros, A.R. et al. (2024) Acta Neuropathol 147, 9.
- Irwin, K.E. et al. (2024) Nat Med 30, 382-393.
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