GPNMB (E7U1Z) Horse Chimeric mAb #28318
- IF
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Horse chimeric IgG4 |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Description
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Frozen) | 1:50 - 1:200 |
Storage
Protocol
Specificity / Sensitivity
GPNMB (E7U1Z) Horse Chimeric mAb recognizes endogenous levels of total GPNMB protein. This antibody does not cross-react with human GPNMB protein.
Species Reactivity:
Source / Purification
The parent antibody, GPNMB (E7U1Z) Rabbit mAb #90205, is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse GPNMB protein.
Background
While typical GPNMB expression is seen in tissues including skin, heart, kidney, lung, liver, and skeletal muscle (3,6), research studies show elevated GPNMB expression often contributes to the metastatic phenotype in numerous cancers (reviewed in 7). GPNMB is typically localized to intracellular compartments in normal cells (1,8), but investigators found it primarily on the cell surface of tumor cells (9,10). Differential localization and expression, and the role of GPNMB as a tumor promoter in many cancer types make this protein a viable therapeutic target (11).
The GPNMB ectodomain can be cleaved by matrix metalloproteinases and shed from the cell surface (12). Research studies identify the sheddase ADAM10 as one peptidase responsible for cleavage of the GPNMB ectodomain at the surface of breast cancer cells. Shedded GPNMB ectodomains may promote angiogenesis by inducing endothelial cell migration (13).
- Tomihari, M. et al. (2009) Exp Dermatol 18, 586-95.
- Sheng, M.H. et al. (2012) PLoS One 7, e35280.
- Shikano, S. et al. (2001) J Biol Chem 276, 8125-34.
- Li, B. et al. (2010) FASEB J 24, 4767-81.
- Patel-Chamberlin, M. et al. (2011) Kidney Int 79, 1138-48.
- Bandari, P.S. et al. (2003) Regul Pept 111, 169-78.
- Maric, G. et al. (2013) Onco Targets Ther 6, 839-52.
- Ripoll, V.M. et al. (2007) J Immunol 178, 6557-66.
- Tse, K.F. et al. (2006) Clin Cancer Res 12, 1373-82.
- Rose, A.A. et al. (2010) Clin Cancer Res 16, 2147-56.
- Keir, C.H. and Vahdat, L.T. (2012) Expert Opin Biol Ther 12, 259-63.
- Furochi, H. et al. (2007) FEBS Lett 581, 5743-50.
- Rose, A.A. et al. (2010) PLoS One 5, e12093.
Limited Uses
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