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  2. Primary Antibodies /
  3. Dab2 (D7O9T) Rabbit mAb (BSA and Azide Free)
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Dab2 (D7O9T) Rabbit mAb (BSA and Azide Free) #98694

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  • WB
  • IF

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 96
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    This product is the carrier free version of product #12906. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

    This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

    BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

    Formulation

    Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

    For standard formulation of this product see product #12906

    Storage

    Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

    Specificity / Sensitivity

    Dab2 (D7O9T) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total Dab2 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly388 of human Dab2 protein.

    Background

    Disabled homolog 2 (Dab2) is a mitogen responsive phosphoprotein that exerts multiple functions through association with numerous proteins. Dab2 modulates signaling pathways through interactions with proteins such as Smads and TGF-β receptors (1,2), axin (3), GRB (4), and Src (5). Dab2 also serves as a cargo-specific adaptor of clathrin-mediated endocytosis via interaction with clathrin (6), AP2 (7), NPXY-containing cargo (8-10), and myosin VI (11,12). In addition, Dab2 regulates cell adhesion by directly binding integrins (13,14). The diverse functions of Dab2 enable it to coordinate cell adhesion, cell motility, membrane trafficking, and signaling. Research studies have shown Dab2 is downregulated in a number of cancers, thereby suggesting a role as a tumor suppressor (15-17). Phosphorylation of Dab2 decreases its endocytotic function (18).
    1. Hocevar, B.A. et al. (2001) EMBO J 20, 2789-801.
    2. Hocevar, B.A. et al. (2005) J Biol Chem 280, 25920-7.
    3. Hocevar, B.A. et al. (2003) EMBO J 22, 3084-94.
    4. Xu, X.X. et al. (1998) Oncogene 16, 1561-9.
    5. Zhou, J. et al. (2003) J Biol Chem 278, 6936-41.
    6. Mishra, S.K. et al. (2002) EMBO J 21, 4915-26.
    7. Morris, S.M. and Cooper, J.A. (2001) Traffic 2, 111-23.
    8. Keyel, P.A. et al. (2006) Mol Biol Cell 17, 4300-17.
    9. Maurer, M.E. and Cooper, J.A. (2006) J Cell Sci 119, 4235-46.
    10. Maurer, M.E. and Cooper, J.A. (2005) J Cell Sci 118, 5345-55.
    11. Morris, S.M. et al. (2002) Traffic 3, 331-41.
    12. Hasson, T. (2003) J Cell Sci 116, 3453-61.
    13. Huang, C.L. et al. (2006) J Cell Sci 119, 4420-30.
    14. Teckchandani, A. et al. (2009) J Cell Biol 186, 99-111.
    15. Mok, S.C. et al. (1998) Oncogene 16, 2381-7.
    16. Anupam, K. et al. (2006) World J Gastroenterol 12, 6041-5.
    17. Bagadi, S.A. et al. (2007) Breast Cancer Res Treat 104, 277-86.
    18. Chetrit, D. et al. (2011) J Biol Chem 286, 5392-403.

    Database Links

    UniProt ID: P98082


    Entrez-Gene Id: 1601


    Limited Uses

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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