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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Cystatin F/CST7 (F9R1W) Rabbit mAb #99376

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  • WB
  • IP
  • IF

    Product Specifications

    REACTIVITY M R
    SENSITIVITY Endogenous
    MW (kDa) 15-25
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Frozen) 1:200 - 1:800
    Immunofluorescence (Immunocytochemistry) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Cystatin F/CST7 (F9R1W) Rabbit mAb recognizes endogenous levels of total cystatin F/CST7 protein.

    Species Reactivity:

    Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with full-length recombinant mouse cystatin F/CST7 protein.

    Background

    Cystatin F, also known as CST7, is an endosomal-lysosomal cysteine protease inhibitor member of the cystatin type 2 superfamily, encoded by the CST7 gene (1). It is selectively expressed by hemopoietic cells such as cytotoxic T cells, natural killer cells, monocytes, and dendritic cells, suggesting a role in immune regulation (1,2). CST7 is expressed as an inactive dimer and converted to a functionally active monomer localized to the endo-lysosomal compartment (3). CST7 has been implicated in various cancer types, including breast and cervical cancer, and is thought to play a role in modulating the tumor microenvironment (4,5). Additionally, CST7 is highly upregulated in disease-associated microglia in Alzheimer’s disease and other neurodegenerative diseases, and may play a sex-specific role in neurodegeneration (6,7).
    For Research Use Only. Not for Use in Diagnostic Procedures.
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