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COL17A1 (F5U1Z) Rabbit mAb #67360

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  • WB
  • IHC

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 190
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:400 - 1:1600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    COL17A1 (F5U1Z) Rabbit mAb recognizes endogenous levels of total COL17A1 protein. This antibody detects a 62 kDa band, likely a processed N-terminal fragment of COL17A1. Non-specific staining was observed in some peripheral nerves, kidney proximal tubules, and small intestine epithelium by immunohistochemistry.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr102 of human COL17A1 protein.

    Background

    Collagen type XVII alpha 1 chain (COL17A1/BP180) is a collagen family hemidesmosome anchorage molecule of basal keratinocytes that promotes stable epidermal-dermal adhesion and wound healing (1). COL17A1 is critical for maintaining keratocyte and hair follicle stem cells, promoting cell motility and increased self-renewing capability (2-4). The protein functions by organizing actin and keratin filament networks via its interaction with intracellular scaffold protein plectin (PLEC) and integrin β4 in the hemidesmosomes (2,5). High expression of COL17A1 is associated with cervical, colorectal, pancreatic, and lung cancers (6-9). It induces a tumor-favorable microenvironment and promotes invasion and metastasis. Conversely, decreased expression of COL17A1 in breast cancer is associated with increased cancer migration and invasion (10). COL17A1 was found to promote cancer dormancy and chemoresistance in colorectal stem cells (11,12). COL17A1 mutations and autoantibodies against COL17A1 can cause blistering skin diseases due to loss of attachment of the epidermis to the underlying basement membrane (13,14).
    For Research Use Only. Not for Use in Diagnostic Procedures.
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