CDK9 (C12F7) Rabbit mAb (BSA and Azide Free) #66968
- WB
- IHC
- IF
- F
Supporting Data
| REACTIVITY | H M R Hm Mk B Dg |
| SENSITIVITY | Endogenous |
| MW (kDa) | 42, 55 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Hm-Hamster
- Mk-Monkey
- B-Bovine
- Dg-Dog
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
For standard formulation of this product see product #2316
Storage
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Cyclin dependent kinases (CDKs) are activated in part by cyclin binding and by phosphorylation of a conserved threonine in the T-loop domain. Phosphorylation of CDK9 at the T-loop Thr186 by an unidentified nuclear kinase may be important in P-TEFb activation (6) and regulation of HIV transcription (7). Acetylation of CDK9 at Lys44 affects its ability to phosphorylate the RNAPII CTD (8).
- Rice, A.P. and Herrmann, C.H. (2003) Curr HIV Res 1, 395-404.
- De Falco, G. and Giordano, A. (2002) Cancer Biol Ther 1, 342-7.
- Shore, S.M. et al. (2003) Gene 307, 175-82.
- Shore, S.M. et al. (2005) Gene 350, 51-8.
- Liu, H. and Herrmann, C.H. (2005) J Cell Physiol 203, 251-60.
- Chen, R. et al. (2004) J Biol Chem 279, 4153-60.
- Ammosova, T. et al. (2005) Retrovirology 2, 47.
- Fu, J. et al. (2007) Mol Cell Biol 27, 4641-51.
Limited Uses
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