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CD11b/ITGAM (E6E1M) Feline Chimeric mAb

CD11b/ITGAM (E6E1M) Feline Chimeric mAb #66973

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Supporting Data

REACTIVITY	M
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Feline chimeric IgG1

Application Key:

IF-Immunofluorescence

Species Cross-Reactivity Key:

M-Mouse

Product Information

Product Description

This Cell Signaling Technology^® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as CD11b/ITGAM (E6E1M) Rabbit mAb #17800.

Product Usage Information

Application	Dilution
Immunofluorescence (Frozen)	1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

CD11b/ITGAM (E6E1M) Feline Chimeric mAb recognizes endogenous levels of total CD11b/ITGAM protein.

Species Reactivity:

Mouse

Source / Purification

This recombinant chimeric antibody is engineered from CD11b/ITGAM (E6E1M) Rabbit mAb #17800 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a feline-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.

The parent antibody, CD11b/ITGAM (E6E1M) Rabbit mAb #17800, is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse CD11b/ITGAM protein.

Background

Cluster of differentiation molecule 11b (CD11b)/Integrin alpha M (ITGAM) is a transmembrane protein forming heterodimers that are composed of α and β subunits (1). CD11b is expressed by, and commonly used as a marker for, myeloid lineage cells, including neutrophils, monocytes, macrophages, and microglia (2). CD11b is phosphorylated at Ser1126 (cytoplasmic tail) in neutrophils. Research has shown that this phosphorylation event plays a role for leukocytes traveling from the bloodstream to tissues (3). Furthermore, genome-wide association studies have linked CD11b to autoimmune diseases, such as systemic lupus erythematosus (SLE) (4).

Database Links

UniProt ID: P05555

Entrez-Gene Id: 16409

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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