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  3. MEK1/2 Control Cell Extracts

MEK1/2 Control Cell Extracts #9160

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    Product Information

    Product Usage Information

    Boil for 3 minutes prior to use. Load 20 µl of phosphorylated and nonphosphorylated MEK1/2 Control Cell Extracts per lane.

    Storage

    Supplied in SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v phenol red or bromophenol blue. Store at –20°C or at –80°C for long term storage.

    Product Description

    Nonphosporylated MEK1/2 Control Cell Extracts: Total cell extracts from HeLa cells, serum starved overnight serve as a negative control. Supplied in SDS Sample Buffer.

    Phosphorylated MEK1/2 Control Cell Extracts: Total cell extracts from HeLa cells, serum starved overnight then treated with 200 nM TPA #4174 for 15 minutes to serve as a positive control. Supplied in SDS Sample Buffer.

    Background

    MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.

    Alternate Names

    lysates; MAPKK-1; MAPKK-2; MEK; MEK-1; MEK-2

    Database Links

    UniProt ID: P36507 , Q02750


    Entrez-Gene Id: 5605 , 5604


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