PathScan® RP ZO-1 Sandwich ELISA Kit #94695
- ELISA
Important Ordering Details
Custom Ordering Details:If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.
Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.
Product Specifications
| REACTIVITY | H Mk |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Description
The rapid protocol (RP) PathScan® RP ZO-1 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of ZO-1 protein in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with ZO-1 in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of ZO-1 protein. Learn more about your ELISA kit options here.
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
Species Reactivity:
Background
- Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35.
- Matter, K. and Balda, M.S. (2007) J Cell Sci 120, 1505-11.
- Hernandez, S. et al. (2007) Exp Cell Res 313, 1533-47.
- Umeda, K. et al. (2006) Cell 126, 741-54.
- Betanzos, A. et al. (2004) Exp Cell Res 292, 51-66.
- Traweger, A. et al. (2003) J Biol Chem 278, 2692-700.
- Huerta, M. et al. (2007) Mol Biol Cell 18, 4826-36.
- Chlenski, A. et al. (2000) Biochim Biophys Acta 1493, 319-24.
Alternate Names
DKFZp686M05161; MGC133289; Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; TJP1; ZO-1; ZO1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1
Limited Uses
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