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PathScan® RP Total Enolase-2 Sandwich ELISA Kit #40806

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  • ELISA

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    Product Specifications

    REACTIVITY H M R
    Application Key:
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Description

    The rapid protocol (RP) PathScan® RP Total Enolase-2 Sandwich ELISA Kit is a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of elonase-2 protein in a reduced assay time of 1.5 hours. Incubation of the cell lysate and detection antibody on the capture antibody-coated microwell plate forms a sandwich with elonase-2 protein in a single step. The plate is then extensively washed, and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of elonase-2 protein. Learn more about all of your ELISA kit options here.

    *Antibodies in this kit are custom formulations specific to the kit.

    Protocol

    Specificity / Sensitivity

    PathScan® RP Total Enolase-2 Sandwich ELISA Kit detects endogenous levels of enolase-2, as shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Human, Mouse, Rat

    Background

    Enolase is a glycolytic enzyme that is involved in the conversion of 2-phosphoglycerate to phosphoenolpyruvate (1). Mammalian enolase has three subunits: α, β, and γ, that can form homo and heterodimers. Homodimers of γ enolase are neuronal-specific (2). Research studies have shown elevated levels of neuro-specific enolase-2 in neuroblastoma (2) and small-cell lung cancer (3,4).

    Alternate Names

    cell based assay; cell-based assay; sandwich elisa; screening

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    PathScan is a registered trademark of Cell Signaling Technology, Inc.
    U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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