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DRAQ7 #7406

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    Product Information

    Product Usage Information

    Flow Cytometry: Prepare cells for DRAQ7™ staining by resuspending cells in PBS at a concentration of no more than 5 x 105 cells/ml. For each 0.5 ml of cell suspension, dilute DRAQ7™ 1:100 by adding 5 μl to suspended cells, achieving a final concetration of 3 μM. Gently mix and incubate for 10 minutes on ice. Cells should be analyzed directly in the presence of DRAQ7™.

    Formulation

    DRAQ7™ is provided as a 0.3 mM stock.

    Storage

    Store at 4°C, protected from light. Do not freeze.

    Fluorescent Properties

    • Exλmax599/644 nm
    • Emλmax678/694 nm
    • DRAQ7™ may be excited by a wide range of wavelengths from 488-647 nm. Despite low absorbance at 488 nm, this excitation source allows for convenient combination with FITC and PE conjugates as well as EGFP.

    Background

    DRAQ7™ is a far-red fluorescent DNA dye that only stains the nuclei in dead and permeabilized cells. It can be used in combination with GFP, FITC, and PE labels.

      Alternate Names

      Cellular dyes; DNA dye; draq

      For Research Use Only. Not for Use in Diagnostic Procedures.
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