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Whitlow/218 Linker (1B4A1) Mouse mAb (Biotinylated) #35016

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  • F

    Product Specifications

    REACTIVITY All
    SENSITIVITY Transfected Only
    MW (kDa)
    Source/Isotype Mouse IgG2a
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is conjugated to biotin under optimal conditions. This biotinylated antibody is expected to exhibit the same reactivity as the unconjugated Whitlow/218 Linker (1B4A1) Mouse mAb #28307, which is expected to react with cell surface-expressed CARs of varying specificity containing a Whitlow/218 linker within the scFv of the extracellular domain.

    Product Usage Information

    Application Dilution
    Flow Cytometry (Fixed/Permeabilized) 1:50
    Flow Cytometry (Live) 1:100

    Storage

    Supplied in 140 mM NaCl, 3 mM KCl, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Whitlow/218 Linker (1B4A1) Mouse mAb (Biotinylated) recognizes exogenously expressed levels of scFv-based CARs containing a Whitlow/218 linker.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of the Whitlow/218 linker peptide.

    Background

    The Whitlow/218 peptide is a commonly used linker that connects the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The Whitlow/218 linker affords the scFv with enhanced resistance to proteolysis and aggregation (1).
    For Research Use Only. Not for Use in Diagnostic Procedures.
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