S100B (E7C3A) Rabbit mAb (Alexa Fluor® 488 Conjugate) #67931
- IHC
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Description
Fluorescent Properties
- ← Excitation: 495 nm ← Emission: 519 nm
Product Usage Information
| Application | Dilution |
|---|---|
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
| Immunofluorescence (Immunocytochemistry) | 1:200 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Each S100 monomer bears two EF-hand motifs and can bind up to two molecules of calcium (or other divalent cations in some instances). Structural evidence shows that S100 proteins form antiparallel homo- or heterodimers that coordinate binding partner proximity in a calcium-dependent (and sometimes calcium-independent) manner. Although structurally and functionally similar, individual members show restricted tissue distribution, are localized in specific cellular compartments, and display unique protein binding partners, which suggests that each plays a specific role in various signaling pathways. In addition to an intracellular role, some S100 proteins have been shown to act as receptors for extracellular ligands or are secreted and exhibit cytokine-like activities (1-4).
S100B is abundantly expressed in astrocytes and is commonly used as an astrocytic marker in studies of the mammalian CNS. S100B is also expressed in immature and mature myelinating oligodendrocytes that are chondroitin sulfate proteoglycan (NG2)-positive (5).
Limited Uses
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