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  3. GITR (D5V7P) Rabbit mAb (SignalFlex™ Alexa Fluor® 555 Conjugate)
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

GITR (D5V7P) Rabbit mAb (SignalFlex™ Alexa Fluor® 555 Conjugate) #82748

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 555 fluorescent dye under optimal conditions and formulated at 200 µg/mL. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated #10419

    Fluorescent Properties

    • ← Excitation: 555 nm ← Emission: 565 nm

    Product Usage Information

    SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.

    Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Specificity / Sensitivity

    GITR (D5V7P) Rabbit mAb (SignalFlex™ Alexa Fluor® 555 Conjugate) recognizes endogenous levels of total GITR protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human GITR protein.

    Background

    TNFRSF18, also known as glucocorticoid-induced tumor necrosis factor-receptor (TNFR)-related protein (GITR) and activation-inducible TNFR family receptor, encodes a type 1 membrane protein of the TNF-receptor superfamily (1). Three alternatively spliced transcript variants encoding distinct isoforms have been reported (2). GITR is an immune cell co-stimulatory receptor expressed constitutively at high levels on CD4+CD25+ T regulatory cells (Tregs), at low levels on naïve and memory T cells, and is induced upon T cell activation (3-5). Studies show GITR can also be induced on NK cells, macrophages, and DCs (3,4,6). Although GITR does not have intrinsic enzymatic activity, TNFSF18 (also known as GITRL) expressed on antigen presenting cells binds to GITR, resulting in recruitment of TNFR-associated factor family members and activation of the NF-κB pathway in T cells (7). GITR ligation has been shown to play a role in CD8+ T cell activation, cytotoxicity, and memory T cell survival (8-10). In the thymus, GITR is thought to play a key role in dominant immunological self-tolerance through thymic Treg differentiation and expansion (11). Of note, GITR ligation inhibits Treg suppressive function (12-13) and promotes effector T cell resistance to Treg suppression (14-15). Due to the combined effects on both Treg suppression and effector cell activation, GITR represents a unique opportunity for immunotherapeutic intervention in cancer (16).
    1. Nocentini, G. et al. (1997) Proc Natl Acad Sci U S A 94, 6216-21.
    2. Nocentini, G. et al. (2000) Cell Death Differ 7, 408-10.
    3. Shimizu, J. et al. (2002) Nat Immunol 3, 135-42.
    4. Nocentini, G. and Riccardi, C. (2009) Adv Exp Med Biol 647, 156-73.
    5. McHugh, R.S. et al. (2002) Immunity 16, 311-23.
    6. Hanabuchi, S. et al. (2006) Blood 107, 3617-23.
    7. Snell, L.M. et al. (2011) Immunol Rev 244, 197-217.
    8. Ronchetti, S. et al. (2007) J Immunol 179, 5916-26.
    9. Kim, I.K. et al. (2015) Nat Med 21, 1010-7.
    10. Snell, L.M. et al. (2012) J Immunol 188, 5915-23.
    11. Petrillo, M.G. et al. (2015) Autoimmun Rev 14, 117-26.
    12. Kanamaru, F. et al. (2004) J Immunol 172, 7306-14.
    13. Valzasina, B. et al. (2005) Blood 105, 2845-51.
    14. Stephens, G.L. et al. (2004) J Immunol 173, 5008-20.
    15. Nishikawa, H. et al. (2008) Cancer Res 68, 5948-54.
    16. Knee, D.A. et al. (2016) Eur J Cancer 67, 1-10.

    Database Links

    UniProt ID: Q9Y5U5


    Entrez-Gene Id: 8784


    Limited Uses

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    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
    SignalFlex is a trademark of Cell Signaling Technology, Inc.
    This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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