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WRN (8H3) Mouse mAb (BSA and Azide Free)

WRN (8H3) Mouse mAb (BSA and Azide Free) #51201

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Supporting Data

REACTIVITY	H M
SENSITIVITY	Endogenous
MW (kDa)	200
Source/Isotype	Mouse IgG1

Application Key:

WB-Western Blotting
IF-Immunofluorescence

Species Cross-Reactivity Key:

H-Human
M-Mouse

Product Information

Product Usage Information

This product is the carrier free version of product #4666. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na₂HPO₄, 3 mM KCl, 2 mM KH₂PO₄, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #4666

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

WRN (8H3) Mouse mAb (BSA and Azide Free) detects endogenous levels of total WRN protein.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with full length human recombinant WRN protein.

Background

The RecQ family of DNA and RNA helicases is a family of enzymes that has been shown to be important to genome integrity (1). Members of this family function in several DNA repair processes, including double-strand break repair, homologous recombination, and re-initiation of DNA replication at stalled replication forks (2,3). Mutations in RecQ helicase family members result in syndromes that display varying types of chromosomal abnormalities and overall genomic instability (1). WRN is a member of the RecQ family that has been identified as the gene underlying Werner’s syndrome, an autosomal recessive disorder characterized by premature aging and predisposition to cancer (4). Cells from Werner’s syndrome patients exhibit genomic instability that is associated with deficient DNA repair activity (5).

Database Links

UniProt ID: Q14191

Entrez-Gene Id: 7486

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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