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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

IκBα (44D4) Rabbit mAb #4812

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  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Hm Mk Mi
    SENSITIVITY Endogenous
    MW (kDa) 39
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Hm-Hamster 
    • Mk-Monkey 
    • Mi-Mink 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #88327.

    Protocol

    Specificity / Sensitivity

    IκBα (44D4) Rabbit mAb detects endogenous levels of total IκBα protein.

    Species Reactivity:

    Human, Mouse, Rat, Hamster, Monkey, Mink

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of human IκBα.

    Background

    The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).

    Alternate Names

    i-kappa-b-alpha; IκB-α; IκBα

    For Research Use Only. Not for Use in Diagnostic Procedures.
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